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RNA Protocols

Preparation of RNA from bacteria (hot-phenol method)
Preparation of RNA from bacteria (phase-lock gel method <--- We're using this these days!!)
Northern blotting (for mRNA)
Northern blotting (for sRNA)
LICOR Northern Blots
Making riboprobes
Ambion's NorthernMax Protocol
Total RNA preparation (from DeRisi Lab, UCSF)
sRNA cloning for 454 sequencing

Other Protocols

Western blotting (chemiluminescence)
Western blotting (Licor)
LICOR Western Blots
Plasmid preps (Qiagen -- large scale)
Plasmid preps (Qiagen -- miniprep)
beta-galactosidase assay
Strategene's Quickchange Mutagenesis Protocol
Preparing electrocompletent cells

Ronak Mistry (CLA '13) works with Prof. Liu to study small RNAs in V. choelrae

Contact Info

Drew University
Department of Chemistry
Hall of Sciences 212
36 Madison Avenue
Madison, NJ 07940

Phone: (973) 408-3303
Fax: (973) 408-3572
jliu3@drew.edu

Last updated: July 27, 2011